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| Author(s): |
Danilo Bueno
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Rio Claro. 2018-08-30. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro |
| Defense date: | 2018-07-27 |
| Advisor: | Henrique Ferreira; Daniele Biscaro Pedrolli |
| Abstract | |
Genetic studies in any organism requires effective tools to change gene expression. Among these, the riboswitches offer a very attractive alternative to modulation of the expression of genes of interest, where it is possible to turn on and turn off such genes without removing them from the genomes. This alternative allows the direct study of a gene and the evaluation of its lack (when disconnected or "off"), as well as it allows the reestablishment without the necessity of the standard complementation (state "ON"). In the search for better conditions of genetic characterization and the development of tools dedicated to studies of essential genes or of difficult characterization in Xanthomonas citri, we evaluate the use of a system of riboswitch theo/metE for the control of gene expression in this bacteria. The characterization itself was performed by altering the expression of the parB and α-amylase genes. The success of these strategies allowed the silencing of parB and α-amylase genes, and it was also possible to observe that the inhibition of the transcription of the α-amylase gene reflected in a decay of the α-amylase enzyme while the riboswitch theo/metE in the presence of his target metabolite, theophylline. The results indicate that the riboswitch theo/metE used in this study is a potential genetic tool for gene silencing in XAC. (AU) | |
| FAPESP's process: | 16/18519-0 - Evaluation of the riboswitch theo/metE for gene silencing in Xanthomonas citri |
| Grantee: | Danilo Bueno |
| Support Opportunities: | Scholarships in Brazil - Master |