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| Author(s): |
Renata Ariza Marques Rossetti
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-06-08 |
| Examining board members: |
Ana Paula Lepique;
Hugo Aguirre Armelin;
Ana Campa;
Eliana Faquim de Lima Mauro;
Marilia Cerqueira Leite Seelaender
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| Advisor: | Ana Paula Lepique; Leandro Gustavo de Oliveira |
| Abstract | |
Cervical cancers have HPV infection as a main factor. HPV tumors recruit leucocytes and change their phenotype as an evasion mechanism. We tried to understand how the tumor influences the immune cells behavior, analyzing signaling pathways important to tumor progression in cervical biopsy and PBMC from patients with high-grade lesions and cancer. We find an increase in NFκB activation in immune cells from tumor microenvironment, but a decrease in PBMC. We could not find any difference in STAT3 signaling pathway in the tumor microenvironment, however, there was an activation increase in PBMC. Both tumor microenvironment immune cells and PBMC had an increase in Akt signaling pathway. To identify a possible strategy to reverse the tumor influence, we stimulated B lymphocytes from patients with sCD40L, achieving an increase in the numbers of CD80+CD86+ cells. Our results demonstrate a tumor effect over the immune cells, with an important systemic effect. However, the approach used to stimulate B lymphocytes from patients present us with a possible immunotherapy. (AU) | |
| FAPESP's process: | 11/11121-8 - Study of signaling pathways triggered by and biological effects of CD40 in cervical tumors. |
| Grantee: | Renata Ariza Marques Rossetti |
| Support Opportunities: | Scholarships in Brazil - Doctorate |