Evaluation of multi-analyte immunoassay technique to quantify sexual steroids in ruminants

Author(s)

Patricia Rotta Lopes

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
• Defense date: 2017-04-18

Examining board members

Claudio Alvarenga de Oliveira; Carla Bargi Belli; Lilian Mara Kirsch Dias; Priscila Viau Furtado; Ana Carolina Gonçalves dos Reis

• Advisor: Claudio Alvarenga de Oliveira

Abstract

Quantification of estradiol and progesterone are used in a big number of animal´s reproduction´s researches. Several methods and antibodies to quantify them have been developed, but each analytical methodology has advantages and limitations. Radioimmunoassay is the most widely used method, but it has as major disavantage the use of radioactive material. The concerns at this area added to the requirements of legislations about environmental topics have increased the need to development techniques without the use of radioactive material. The aim of this research was to evaluate the xMAP-Multiplex® technique to quantify progesterone and estradiol in ovines, bovines and bubalins. Five animals of each species were used. The estrous cycle of the animals was synchronized by prostaglandin and blood samples were collected during the estrous cycle. The seric concentrations of progesterone and estradiol were obtained by MILLIPLEX® MAP Multi Species Steroid kit (STTHMAG-21K; Millipore, Billerica, MA). Under the conditios of this experiment the results showed that this technique is practicable to evaluate progesterone´s profile in those animals, but not to evaluate their estradiol´s profile. (AU)

• FAPESP's process: 13/16755-0 - Evaluation of multi-analyte immunoassay to quantify sexual steroids in domestic animals.
• Grantee: Patricia Rotta Lopes
• Support Opportunities: Scholarships in Brazil - Doctorate