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| Author(s): |
Bárbara Tavares Schäfer
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2018-03-28 |
| Examining board members: |
Ii Sei Watanabe;
Fernando José Dias;
Silvia Lacchini;
Edson Aparecido Liberti;
Simone Cristina Motta
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| Advisor: | Ii Sei Watanabe |
| Abstract | |
The White-eared Opossum (Didelphis albiventris) is a marsupial that occupies a wide variety of habitats, ranging from forests to urban areas, in several Brazilian biomes and plays an important role in the dissemination of seeds. The objective of this research is to study the lingual morphology of this species. The tongues were collected from animals found dead on roads in the state of Rio Grande do Sul or by cadaver donation from the Núcleo de Reabilitação da Fauna Silvestre (NURFS / UFPel). The samples were submitted to light and scanning (SEM) and transmission (TEM) electron microscopy techniques. Through light microscopy we observed the epithelium stratified squamous and the budding of the papillae in fetuses, the development of the lingual papillae in infants and the characteristics of the fully developed papillae in adults. By the staining of Azan and Picrosirius we observed that the collagen is located mainly under the epithelium. Through the SEM the analysis of the epithelial layer revealed three filiform papillae with different morphologies according to the region. The fungiform papillae are scattered at the apex and body lingual, and partially covered by an elevation of mucosa. Circumvallate papillae are 3 in number and form a triangle at the lingual root. Foliate papillae are small and slender elevations in the caudolateral border of the tongue, in varying numbers of 13-18 projections. The subepithelial tissues of fungiform, circumvallate and foliate papillae have an irregular surface with some dorsal depressions. Through MET, the stratum corneum demonstrated, for adults and fetuses, the process of epithelial renewal through the release of the most superficial cells; in the granular layer we observed tonofilaments and granules of kerato-hyaline; The spinous layer was similar in both ages and was marked by the great amount of intercellular desmosomes. The characteristics previously observed in the tongues of D. albiventris are similar to those observed in other species of marsupials. The experimental procedures were approved by the Committee on Ethics in the Use of Animals (CEUA / FMVZ / USP). (AU) | |
| FAPESP's process: | 15/05065-9 - Observation about the lingual mucosa of the White-Eared-Opossum using light microscopy and scanning and transmission electron microscopy method |
| Grantee: | Bárbara Tavares Schäfer |
| Support Opportunities: | Scholarships in Brazil - Doctorate |