Influence of the amount of dentifrice and fluoride concentration on intraoral fluoride uptake and on fluoride intake by children: in vivo study

Author(s)

Caio Sampaio

• Document type: Master's Dissertation
• Press: Araçatuba. 2018-12-04.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2018-11-26
• Advisor: Juliano Pelim Pessan; Alberto Carlos Botazzo Delbem

Abstract

The use of fluoridated dentifrices is regarded as the best vehicle of fluoride (F) use, since it combines the mechanical removal of biofilms with the therapeutic effects of F. Nevertheless, the evidence for recommending such products to children are still inconsistent, given the balance between dental caries and fluorosis. Thus, the aim of the present study was to evaluate F concentrations in saliva of toddlers after brushing with dentifrices containing different F concentrations, applied in different quantities, as well as to estimate F intake from toothbrushing. Toddlers (n=18, 2-3 years old) were randomly assigned into six experimental groups, according to the possible combinations of dentifrices (0/550/1100 ppm F, as NaF) and amount applied on the toothbrush (0.04/0.16/0.32 g, corresponding to rice grain, pea-size and using the transverse technique, respectively). Volunteers used a placebo dentifrice during one week. On the 7th day, saliva samples were collected before (baseline), and at 5, 15, 30 and 60 minutes after toothbrushing with one of the possible treatment combinations. All toothpaste expectorated after brushing was collected. F concentrations were determined after buffering with TISAB III (saliva) and hexamethyldisiloxane-facilitated diffusion (expectorate). Data were submitted to ANOVA or Kruskal-Wallis test, followed by Fisher’s LSD or Student-Newman-Keuls’ tests, respectively (p<0.05). Brushing with the 550 ppm F toothpaste (pea-size or transversal technique) increased AUC values at similar levels to those attained by brushing with 1100 ppm F (rice grain). The highest AUC and salivary F at 5 min after brushing were achieved by brushing with 1100 ppm F (pea-size), followed by the 550 ppm F (transversal technique). As for F intake during toothbrushing, the highest values were observed for the 550 ppm F (transversal technique), followed by 1100 ppm F (pea-size). It was concluded that the amount of toothpaste and F concentration in the product significantly affected both salivary F concentrations and F intake during toothbrushing. (AU)

• FAPESP's process: 17/07424-1 - Influence of the amount of dentifrice and fluoride concentration on intraoral fluoride uptake and on fluoride intake by children: in vivo study
• Grantee: Caio Sampaio
• Support Opportunities: Scholarships in Brazil - Master