Influence of the amount of dentifrice and fluoride concentration on intraoral fluoride uptake and on fluoride intake by children: in vivo study

• Grant number: 17/07424-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2017
• End date: November 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Juliano Pelim Pessan
• Grantee: Caio Sampaio
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This study aims to evaluate fluoride (F) concentrations in saliva, as well as analyze F intake after brushing with dentifrices containing different fluoride concentrations (0, 550 e 1100 ppm F, neutral pH), applied on different quantities (approximately 0,04, 0,16 e 0,32 g). Volunteers (n=24, 2-3 years old) will be randomly assigned into 6 experimental groups, according to the possible combinations of dentifrices and quantities. The children will use a placebo dentifrice during the week. On the 7th day, samples of unstimulated saliva will be collected before brushing (baseline), and 5, 15, 30, 60 e 120 minutes after brushing with one of the possible treatment combinations (amount of dentifrice x F concentration in product), following double-blind, crossover protocol. The amount of fluoride ingested during toothbrushing will be also assessed, through the analysis of fluoride concentration present in expectorant and remaining on the toothbrush (which will be subtracted from the amount of F initially placed on the toothbrush). Samples will be analyzed with an ion-specific electrode after HMDS-facilitated diffusion. The results will be analyzed for normality (Kolmogorov-Smirnov) and homogeneity (Barlett); if the use of parametric tests is possible, data will be analyzed by ANOVA and Tukey's test. Otherwise, non-parametric tests will be used. A significance level of 5% will be assumed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAMPAIO, CAIO; DELBEM, ALBERTO CARLOS BOTAZZO; PAIVA, MAYRA FRASSON; ZEN, IGOR; DANELON, MARCELLE; CUNHA, ROBSON FREDERICO; PESSAN, JULIANO PELIM. Amount of Dentifrice and Fluoride Concentration Influence Salivary Fluoride Concentrations and Fluoride Intake by Toddlers. Caries Research, v. 54, n. 3, p. 234-241, OCT 2020. (17/07424-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SAMPAIO, Caio. Influence of the amount of dentifrice and fluoride concentration on intraoral fluoride uptake and on fluoride intake by children: in vivo study. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.