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| Author(s): |
Marília Domingues de Andrade
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2015-11-12 |
| Examining board members: |
Leonilda Maria Barbosa dos Santos;
Clarissa Lin Yasuda;
Rodolfo Thomé
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| Advisor: | Leonilda Maria Barbosa dos Santos |
| Abstract | |
Neuromyelitis optica (NMO) is an autoimmune demyelinating disease of the central nervous system. Although about 20% of patients do not exhibit anti aquaporin 4 (AQP4 antibodies, there is evidence that tissue damage is more severe in patients with autoantibodies to AQP4. In this study, we show that the positivity of anti AQP4 antibodies in the serum of patients with NMO was above 80%. Since B-lymphocytes give rise to plasma cells producing antibodies, we aimed to study different populations of B-lymphocytes in peripheral blood and cefalorraquiano fluid of patients with NMO. The percentages of naïve (CD27-IgD+), conventional (CD27+IgD-) and double negative memory (CD27-IgD-) B-lymphocytes were determined by flow cytometry. There was no significant difference between the group of patients with NMO and normal subjects. We also assessed by flow cytometry IL-10 production by B cells. We observed a higher frequency of B-lymphocytes producing IL-10 in patients in comparison to the group of healthy individuals (AU) | |
| FAPESP's process: | 13/00640-0 - Interaction between T lymphocytes, Blymphocytes and plasmacytoid dendritic cells in patients optical neuromyelitis |
| Grantee: | Marília Domingues de Andrade |
| Support Opportunities: | Scholarships in Brazil - Master |