Analysis and development of bioinformatics protocols for epigenetics studies

Author(s)

Wélliton de Souza

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas
• Defense date: 2016-01-26

Examining board members

Íscia Teresinha Lopes Cendes; Francisco Pereira Lobo; Samara Flamini Kiihl

• Advisor: Íscia Teresinha Lopes Cendes

Abstract

Epigenetic changes are those that occur during cellular division, modifying gene expression without changes on the DNA sequence. They are involved in gene silencing, tissue differentiation and in molecular mechanisms of a number of phenotypes. DNA methylation, an epigenetic modification that changes chromatin, has been studied extensively in various normal and pathologic biological processes. With the advancement of methods and technologies for DNA sequencing, it is now possible to perform analysis to determine methylation profiles and their association with phenotypes of interest. This dissertation adds the analytical perspective of epigenetics related to studies of animal models of epilepsy. Protocols were defined in bioinformatics processing of DNA methylation data. Methylation profiles were determined and associated with epilepsy phenotypes of animals studied, which also allowed the identification of opportunities for the development of new tools and automation of defined protocols (AU)

• FAPESP's process: 13/24801-2 - Analysis and Development of Protocols in Bioinformatics for Epigenetic Studies
• Grantee: Wélliton de Souza
• Support Opportunities: Scholarships in Brazil - Master