The role of Platelet-Rich plasma on the development of atherosclerosis disease in mice model

Author(s)

Letícia Queiroz da Silva

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas
• Defense date: 2018-01-30

Examining board members

Joyce Maria Annichino Bizzacchi; Erich Vinicius De Paula; Rita de Cassia Aleixo Tostes Passaglia

• Advisor: Joyce Maria Annichino Bizzacchi

Abstract

Atherosclerosis is a chronic, multifactorial inflammatory disease, slow and progressive, characterized by the formation of atheroma inside of blood vessels, which is the main cause of worldwide mortality. It is well known that the Platelet Rich-Plasma (PRP) presents high levels of growth factors and other components that triggers to a therapeutic efficiency, as some studies have been demonstrating. Moreover, it has been proved another platelet function related to immunoregulation. The aim of this study was to evaluate the effect of PRP in the development of the atherosclerosis disease in knockout mice model for the lipoprotein receptor (LDLr -/-). The animals were fed with a high fat diet (HF). It was analyzed the functional activity of fresh and lyophilized PRP of humans by the metabolic activity test, and the results allow us to choose the lyophilized PRP as a therapeutic option for the mice model. The animal model of atherosclerosis was evaluated through weight, cholesterol and triglyceride serum levels, transformer-ß growth factor (TGF-ß) levels and several cytokines; Our data showed that it was possible to concentrate 3.2 folds the number of platelets in relation to the baseline count, which is characterized as a PRP product. The same was found in TGF-ß levels in lyophilized PRP and the maintaining of such a growth factor is important for a better therapeutic effect, however it was not significant. In relation to the development of the disease in mice, we observed an increase in triglyceride levels (58.6 vs. 110.0 mg / dL, p = 0.089) and cholesterol (172.0 vs. 249.5 mg / dL, p = 0.0013), in animals with normal diet or HF, respectively. The weight of the HF-fed animals was 25.5 and 18.5 g in the normal diet group (p <0.001). The cholesterol and triglyceride levels, in the mice treated with PRP for 1 month, were lower than in the control group: 190.4 vs. 216.1 mg / dL (p = 0.490) for cholesterol and 195.9 vs. 82.8 mg / dL (p = 0.05) for triglycerides. However, after 2 months of application, cholesterol levels were similar: 214.7 in the serum vs. control group amd 190.1 mg / dL in the PRP group (p = 0.419)... The abstract is available with the full electronic document (AU)

• FAPESP's process: 16/00356-8 - The effect of platelet-rich plasma on atherosclerosis development in mice animals
• Grantee: Letícia Queiroz da Silva Justo
• Support Opportunities: Scholarships in Brazil - Master