Biopharmaceutical approach of polymer particles in infection-causing bacteria in the urinary tract

Author(s)

Danilo Antonini Alves

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2016-11-28

Examining board members

Marcelo Lancellotti; Rodrigo Ramos Catharino; André Lisboa Rennó; Daniel Fábio Kawano; Luciano Moura Martins

• Advisor: Marcelo Lancellotti

Abstract

A urinary tract infection (UTI) is a common disorder characterized by the presence of microorganisms in the urinary system. These diseases are responsible for much of the infectious processes acquired in the community and in hospital settings. Escherichia coli, Pseudomonas aeruginosas and Klebsiella pneumoniae are described in the literature as the main responsible for the causes of UTI. Indiscriminate use of antibiotics over time, caused pathogenic microorganisms would create adaptive mechanisms of resistance to several existing drugs, becoming the subject of concern of public health agencies, especially as it relates to hospital settings. This work will aim to propose new treatment alternatives for patients with UTI based on polymer particles associated with antimicrobial agents of the quinolone class. These particles were characterized by the techniques of Dynamic Light Scattering , Nanoparticle Tracking Analysis and Zeta potential. The dissolution profile and release of systems obtained was evaluated by vertical diffusion technique in vitro Franz cell. The biological evaluation of the compounds was evaluated by the Minimum Inhibitory Concentration technique using bacteria isolated from patients with urinary tract infection and evaluation of cytotoxicity by cell viability assay Through MTT reduction (AU)

• FAPESP's process: 11/21822-3 - Mechanisms of antibiotic resistance aquisition by bacteria causing infection of human urinary tract - a farmacogenomic and biopharmaceutic design
• Grantee: Danilo Antonini Alves
• Support Opportunities: Scholarships in Brazil - Doctorate