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| Author(s): |
Bárbara Donadon Reina
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Araraquara. 2019-06-06. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara |
| Defense date: | 2019-05-08 |
| Advisor: | Lívia Nordi Dovigo |
| Abstract | |
It is estimated that more than 60% of human infections are originated by the presence of biofilms. The dental plaque, reported as the oldest biofilm, can be composed of many different species, highlighting Lactobacillus spp, Streptococcus mutans and Candida albicans. Photodynamic Inactivation (PDI) has been suggested as a promising therapeutic modality for the inactivation of oral microorganisms, in order to overcome the problems related to microorganism resistance and prevent adverse side effects. Previous studies have shown that curcumin is a promising photosensitizer for PDI. Nevertheless, curcumin has low solubility in aqueous solutions, therefore, this study proposed to evaluate the antimicrobial efficacy of the PDI, using the curcumin in a liquid crystal precursor system (LCPS), in dental biofilms formed in situ. This system has several advantages described in the literature, such as its bioadhesiveness at the site of action, controlled release and biocompatibility. However, no study evaluated the LCPS carrying curcumin for photodynamic action on biofilms formed in situ. This study included 30 volunteers who used oral palatal devices containing bovine enamel blocks for 48 hours for in situ biofilm formation. After that period, the enamel blocks were randomized into two groups: i. control group (empty LCPS only); ii. PDI (immersed in LCPS with curcumin in the dark and then illuminated with a blue light LED) incubated, (PDI group). After the treatments, the CFU/ml of each group was determined in culture media presumptive for Streptococci mutans, Candida spp, Lactobacillus spp. and aciduric species, and total microbiota, specific to the culture conditions for this study. Multivariate analysis showed that the treatment had a large and highly significant effect on microbial viability. Univariate ANOVA was then performed, which revealed a significant reduction on log10(CFU/mL) values in the PDI groups, compared to the control group, for all the culture conditions. Mean reductions were obtained in the order of 2,08; 1,34; 1,80 e 1,54 log10 for Chromagar Candida, MRS, Müller Hinton e Mitis Salivarius respectively. In addition, the descriptive data suggest that there was some variation of the effect of the PDI between different individuals. This fact was confirmed by the results of hierarchical modeling analysis. In conclusion, curcumin-mediated photodynamic inactivation in LCPS was effective in reducing the viability of microorganisms present in in situ biofilms, which seems to be a promising technique for the inactivation of dental biofilms and for future clinical studies. (AU) | |
| FAPESP's process: | 16/24918-5 - Efficacy of curcumin-mediated photodynamic inactivation against in situ dental biofilms |
| Grantee: | Bárbara Donadon Reina |
| Support Opportunities: | Scholarships in Brazil - Master |