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| Author(s): |
Joyce Regina Zapaterini Rossi
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2017-04-17. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu |
| Defense date: | 2017-02-24 |
| Advisor: | Luis Fernando Barbisan; Wagner José Fávaro |
| Abstract | |
The breast cancer has a diverse natural history, complex histology, and its incidence has been increased in the last decades. Actually, there are few treatments against hormone-dependent breast cancer and the drugs commonly used generate serious side effects. The immunotherapy represents a new perspective to cancer treatment and researches about the role of toll-like receptors in carcinogenesis have been increased. In this context, P-MAPA is an important immunomodulatory, which has effectively demonstrated antitumor activity by increasing cytokines expression and by stimulating lymphocytes and toll-like receptors. The aims of this study were to characterize and compare the histopathological and molecular effects of P-MAPA immunotherapy with estrogenic receptor blocker (Tamoxifen) in the treatment of breast cancer chemically-induced in rats. For this purpose, female Sprague-Dawley rats were allocated into six experimental groups: the groups 1-4 received a single dose of 7,12- dimetil-benzoantracene (DMBA) carcinogen (80 mg/kg, i.g) in the first experimental week, while the groups 5-7 received a single dose of DMBA vehicle. After, groups 2 and 5 received P-MAPA doses (5,0 mg/kg, 3x/week, i.p) and vehicle doses of Tamoxifen (5x/week, s.c). The groups 3 and 6 received P-MAPA doses and Tamoxifen doses (100µg/kg, 5x/week, s.c) and the group 4 received Tamoxifen doses and P-MAPA vehicle doses. The control groups (1 and 7) received the P-MAPA and Tamoxifen vehicles doses. Animals were euthanized at the end of the 13th week. The normal mammary and tumoral tissues, spleen, liver and kidneys were removed and subjected to histopathological, immunohistochemical and molecular analysis. (AU) | |
| FAPESP's process: | 13/15060-9 - Histophatological and Molecular Analysis of P-MAPA Immunotherapy with Tamoxifen in Chemically Induced Breast Cancer Progression in Sprague-Dawley Rats. |
| Grantee: | Joyce Regina Zapaterini Rossi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |