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| Author(s): |
Analú Barros de Oliveira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Araraquara. 2020-03-30. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara |
| Defense date: | 2020-03-04 |
| Advisor: | Fernanda Lourenção Brighenti; Carla Raquel Fontana Mendonça |
| Abstract | |
This study is divided into 2 publications whose objectives were: a) to carry out a meta-analysis about antimicrobial photodynamic therapy (PDT) in Denstistry (Publication 1) and b) To evaluate the in vitro potential of essential oils of Coffea arabica, Matricaria Recutita and Eugenia uniflora and plant extracts of Senna Splendida, Senna Reticulata and Senna Macranthera for use as monotherapy in photodynamic therapy on suspensions of microorganisms of medical and dental interest. Publication 1: A research question and how keywords were constructed according to the PICO strategy. A search of the article was carried out in the Embase, Lilacs, Scielo, Medline, Scopus, Cochrane Library, Web of Science, Science Direct and Pubmed databases. Randomized controlled trials and in vitro studies were selected in the review. The study was conducted according to the PRISMA guidelines for systematic review. Publication 2: The following references strains were used in suspension: Cutibacterium acnes ATCC 6919, Streptococcus mutans ATCC 35688, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans ATCC 90028. The plant materials were tested at 50 μg / mL (extracts) or 2% (essential oils). Five groups were studied: negative control, plant material without exposure to light (FS-light), plant material with exposure to light (FS + light), vehicle control without exposure to light (CV-light) and vehicle control with exposure the light (VC + light). The effectiveness of the therapy was assessed by counting viable cells after treatment. The production of reactive oxygen species was evaluated in plant materials that showed the best antimicrobial activity. For this test, 3′-p- (aminophenyl) fluorescent probes (APF; mainly detects the radical hydroxyl [• OH]) and the Singlet Oxygen Sensor (SOSG; detects singlet oxygen [O2]) were used. The data were analyzed using the IBM SPSS version 20.0 program, with a significance level of 5%. For C. acnes and S. mutans, total microbial reduction was observed in 2 of the 6 plant materials tested. For S. aureus and C. albicans, total microbial reduction was observed in 4 of the 6 plant materials tested. A total reduction of 1 out of 6 natural materials was observed for E. coli. S. macranthera and S. reticulata were produced the greatest amount of reactive oxygen species, followed by S. splendida, E. uniflora and M. recutita. In conclusion, Publication 1 highlights that there is no consensus on the study protocols for PDT against cariogenic microorganisms, although the results shown that a PDT may be a good alternative for the treatment of dental caries. Publication 2 shows that antimicrobial photodynamic therapy (PDT) mediated by different natural substances was efficient in reducing microbial suspensions of microorganisms of medical and dental interest. (AU) | |
| FAPESP's process: | 18/18440-0 - Bioprospection of natural products in photodynamic therapy against microorganisms of medial and dental importance |
| Grantee: | Analú Barros de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Master |