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| Author(s): |
Natalia Gomes Vieira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2018-12-10 |
| Examining board members: |
Jorge Mauricio Costa Mondego;
Douglas Silva Domingues;
Luiz Filipe Protasio Pereira;
Maria Helena de Souza Goldman;
Renato Vicentini
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| Advisor: | Jorge Mauricio Costa Mondego |
| Abstract | |
Coffee is one of the most important agricultural commodities in the world. As an alotetraploid, Coffea arabica genome is the merger of its diploid ancestors¿ genome (Coffea canephora and Coffea eugenioides) which became subgenomes in this species. Therefore, transcripts expressed by C. arabica are a combination of homelogous genes of these two subgenomes. The regulation of these genes modulates expression in different tissues and biological conditions, which could explain the greater environmental plasticity of C. arabica and others polyploids comparing to diploid plants. Thus, the aim of our study was to evaluate Frigida-like family (FRLs) and ethylene receptor EIN4 gene, observing the differences in gene sequences and promoters, as well as investigating homeolog differential expression (HDE) throughout reproductive development and somatic embryogenesis in C. arabica. RNA from leaves, flowers, fruits and embryos at different stages of development was extracted and qPCR assays were performed using Taq-MAMA method to discriminate homeolog sequences. Our work is one of pioneers to use C. canephora and C. arabica complete genome data and C. eugenioides transcriptome data to study homeolog genes and for the first time Frigida-like orthologs were identified and characterized in Coffea. Surprisingly, in addition to confirming FRL gene expression during zygote embryogenesis, we detected FRL expression during direct somatic embryogenesis. Regarding EIN4, it was more expressed in embryo than others tissues on fruit and there was an increase in expression in the final stages of development in the endosperm and pericarp. In addition, FRLs and EIN4 homeologous were differentially expressed. We also evaluated whether methylation or the presence/absence of cis elements in homeologous promoters could explain HDE regulation. We considered our work an important step for elucidate HDE regulation of C. arabica, supporting alternative strategies for breeding and guided selection (AU) | |
| FAPESP's process: | 13/17544-3 - Identification of DNA methylation regulating the differential expression of homeologous genes in Coffea arabica |
| Grantee: | Natalia Gomes Vieira van den Broek |
| Support Opportunities: | Scholarships in Brazil - Doctorate |