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| Author(s): |
Luciana Moreira Martins
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-12-13 |
| Examining board members: |
Ronaldo Zucatelli Mendonça;
José Antonio Jerez;
José Luiz Caldas Wolff
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| Advisor: | Ronaldo Zucatelli Mendonça |
| Abstract | |
The protein expression system using baculovirus as a vector has been intensively used for the production of recombinant proteins because it allows the formation of post-translational modifications and has a strong promoter, polyhedrin. However, in some cases, this expression system is not as effective because the infection of cells by baculovirus induces death by apoptosis, thereby limiting the industrial production of various recombinant proteins of interest. We report the occurrence of apoptosis in cultured insect cells and mammalian depleted of nutrients or induced by chemical and viral agents, and supplementation of these cultures with hemolymph of Lonomia obliqua is able to extend the viability of the culture avoiding death by apoptosis. This study aimed to determine in advance the anti-apoptotic mechanism of action of a protein isolated from hemolymph of L. obliqua. The identification of this mechanism may help develop strategies for controlling apoptosis in cell culture allowing the optimization of productivity of viral and recombinant proteins. (AU) | |
| FAPESP's process: | 09/06511-1 - Study of protein expression in cells treated with an antiapoptotic protein, derived from Lonomia oblique hemolymph |
| Grantee: | Luciana Moreira Martins |
| Support Opportunities: | Scholarships in Brazil - Master |