Evaluation of cytotoxic and melanogenic activity of the complex of platinum (II) with hydantoin derivative in melanoma.

Author(s)

Fernanda Branco Filippin

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ)
• Defense date: 2013-09-11

Examining board members

Silvya Stuchi Maria Engler; Carmen Verríssima Ferreira; Ana Paula de Melo Loureiro

• Advisor: Silvya Stuchi Maria Engler

Abstract

Since melanoma is one of the most aggressive forms of skin cancer and more resistant to conventional treatments, the present study aimed to evaluate the activity of a new platinum complex (II) with hydantoin derivative (CX42) in melanoma cells human and murine. Skin cells (fibroblasts, keratinocytes and melanocytes) were used as control and the compounds cisplatin (CIS) and hydantoin compound alone (NN10) were also evaluated. Viability assays, cell cycle analysis and cell death characterization were performed. Likewise, the activity of tyrosinase, the key enzyme that regulates melanin synthesis during the process known as melanogenesis, was also investigated. The results demonstrated a reduction in cell viability and cell cycle arrest in G0/G1 phase of murine melanoma B16F10 treated with CX42. In B16F10 cells, it was also observed melanogenesis stimulation with increased activity of tyrosinase. The CX42 showed a selective cytostatic effect on melanoma cells, however no toxic effects were observed in skin cells. In addition, with prior stimulation of melanogenesis, the CX42 demonstrated to be more effective, increasing apoptosis cell death in 40%. Therefore, the results demonstrated that CX42 decreased cell viability and the intensity of its effect is associated with cell pigmentation, demonstrating an association between tyrosinase high activity and induction of cell death which is an important attribute for new potential anti - tumoral drugs. (AU)

• FAPESP's process: 11/15729-0 - Melanogenic Activity of Platinun (II)Complex with Derivates of Hydantoines as a New Route of Cytotoxicity in Melanoma
• Grantee: Fernanda Branco Filippin
• Support Opportunities: Scholarships in Brazil - Master