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| Author(s): |
Tatiane Marques Porangaba de Oliveira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-04-20 |
| Examining board members: |
Marcelo Palma Sircili;
Joao Carlos Monteiro de Carvalho;
Sylvia Luisa Pincherle Cardoso Leão
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| Advisor: | Marcelo Palma Sircili |
| Abstract | |
C-phycocyanin (C-PC) is a water soluble pigment and is present in Arthrospira platensis. It is consisted of two subunits, α and β, with molecular masses of 16 and 17 kDa, respectively. Recently it was shown that the β subunit of C-PC Anabaena has antitumor activity. The main objectives of this study were cloning and expression the β subunit of C-PC (β-PC) of A. platensis in Escherichia coli and check the ability of the C-PC and recombinant proteins in apoptotic induction in cancer (HEp-2) and non-cancer (fibroblasts) cells. The fibroblasts and HEp-2 cells were treated with 100 µg of recombinant and C-PC, proteins respectively for 6 h. After treatment, the cells were stained with toluidine blue (Critical Electrolyte Concentration Method - CEC). Apoptosis cells were detected by staining and morphological changes. The results show that the recombinant β-PC and C-PC proteins are able to induce apotosis in HEp-2 cells and dont induce it in fibroblasts cells. (AU) | |