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Characterization of membrane proteins of Leptospira interrogans expressed in Escherichia coli.

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Author(s):
Renata de Siqueira Mendes
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Ana Lucia Tabet Oller do Nascimento; Waldir Pereira Elias Junior; Irene da Silva Soares
Advisor: Ana Lucia Tabet Oller do Nascimento
Abstract

The genomic sequencing of the L. interrogans serovar Copenhageni and the advances of bioinformatics analysis allowed the identification of six new vaccine candidates. These genes were subjected to genomic DNA, mRNA and native protein conservation assays in twelve serovars from Leptospira, and the gene LIC11469 was the most conserved among these serovars. The recombinant proteins rLIC11469 and rLIC11030 were purified by metal affinity chromatography, and subjected to circular dichroism. Through cellular localization assays we could observe the presence of the corresponding native proteins on the outer membrane of Leptospira. In adhesion assays, the protein rLIC11469 showed binding to laminin and plasminogen. Immunization and challenge assays showed that the protein rLIC11030 afforded protection against lethal leptospiral inoculation in hamsters. Both proteins showed reactivity against sera of patients diagnosed with leptospirosis, suggesting that these proteins are probably expressed during infection. (AU)

FAPESP's process: 09/02316-0 - Characterization of membrane proteins of Leptospira interrogans expressed in Escherichia coli
Grantee:Renata de Siqueira Mendes
Support Opportunities: Scholarships in Brazil - Master