Study of metanotrophic community present in sediment samples from Bertioga´s mangrove, State of São Paulo, using nucleic acid labeling with stable isotope probing technique (DNA-SIP)

Author(s)

Débora do Carmo Linhares

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2012-05-02

Examining board members

Vivian Helena Pellizari; Fernando Dini Andreote; Claudia Echevengua Teixeira

• Advisor: Vivian Helena Pellizari

Abstract

Methanotrophic bacteria are able to use methane as sole carbon and energy source, and its importance in the environment is related to the mitigation of methane emissions to the atmosphere. Brazilian mangroves are highly productive, have potential to methane production, and it is inferred that methanotrophic community is of great importance for this ecosystem. The scope of the project was to investigate the functional and taxonomic diversity of methanotrophic bacteria present in sediments from Bertioga´s mangrove (SP, Brazil), through new techniques of cultivation and enrichment, including the technique of DNA-SIP with the use of methane as a labeled substrate, which allows to study the role of non-culturable bacteria in the oxidation of CH4. Microbial consortia using methane for growth were obtained from studied samples. Representing the active microbiota (labeled DNA), were identified classic metanotrophs belonging to Family Methylococcaceae, and groups of micro-organisms whose role in methane cycle is uncertain, as the Family Methylophilaceae and the Phylum TM7. (AU)

• FAPESP's process: 09/06601-0 - Characterization of methanotrophic community and its isolates from Bertioga´s mangroves, São Paulo
• Grantee: Débora Do Carmo Linhares
• Support Opportunities: Scholarships in Brazil - Master