Full text
| |
| Author(s): |
Lygia Samartin Gonçalves Luchini
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-05-12 |
| Examining board members: |
Denise Vilarinho Tambourgi;
Carla Cristina Squaiella Baptistão;
Anderson de Sá Nunes
|
| Advisor: | Denise Vilarinho Tambourgi |
| Abstract | |
The snake venom of Bothrops genus (responsible for 80% of envenomation in Brazil) is composed by metallo and serine proteases, disintegrins, phospholipase, among others, and can cause edema, hemorrhage, necrosis, and systemic manifestations, such as intravascular coagulation, shock, renal failure and systemic hemorrhage. The venom of B. pirajai is able to activate the complement system (C), suggesting a contribution to the worsening of symptoms. Considering the importance of C in the inflammatory process and the role of metalloproteinases in envenomation, it was found that treatment of human whole blood (where cells and plasma mediators interact) with or without compstatin (C3 inhibitor), and with a class PI metalloproteinase from B. pirajai\'s venom led to highly significant differences in the expression of the markers analyzed in leukocytes, in generation of anaphylatoxins and TCC, and quantification of cytokines and chemokines in plasma, suggesting that inhibition of C reduces the inflammatory process and may be an effective therapy for bothropic envenomations. (AU) | |
| FAPESP's process: | 13/26198-1 - Role of the complement system in the inflammatory process caused by Bothrops jararaca venom: analysis in the ex-vivo model of human whole blood |
| Grantee: | Lygia Samartin Gonçalves Luchini |
| Support Opportunities: | Scholarships in Brazil - Master |