Screening newly microbial strains for tannase and lipase production
Screening of newly yeasts from Sao Paulo regions as potencial producers of lipase ...
Bioprospection, production, purification, immobilization and characterization of f...
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Screening newly microbial strains for tannase and lipase production
Full text
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| Author(s): |
Érica Benjamim da Silva
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos |
| Defense date: | 2014-04-30 |
| Examining board members: |
Gabriela Alves Macedo;
Danielle Branta Lopes;
Luciana Francisco Fleuri
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| Advisor: | Gabriela Alves Macedo |
| Abstract | |
The bioprospection of native microorganisms capable of producing enzymes with diverse catalytic properties is essential to the development of the Brazilian biotechnological sector. Lipase is an enzyme with wide biotechnological application, it is used on chiral drugs resolution, fat modification and on the production of biofuels, cosmetics and flavor enhancers. Tannase is another enzyme with large potential to be applied at industries, it is capable of catalyze the hydrolysis of hydrolysable tannins and galic acid esters. Therefore, this work goal is to isolate novel microorganisms strains capable of producing extracellular lipase and tannase, and to characterize biochemically the selected strains. 131 fungal strains and 109 yeast strains were isolated from Amazon and Atlantic Rainforest regions. These fungal strains summed with others strains from the Microorganisms Collection of the Biochemistry Laboratory/ FEA-UNICAMP (n=348) by differential solid media. It was found 26 lipase producing strains and 105 tannase producing strains. The positive strains were evaluated regarding their capacity to produce lipase and tannase by solid state fermentation. Tannase¿s enzymatic activity was measured by spectrophotometry and lipases¿s enzymatic activity by neutralization titration. Lipase from Colletotrichum sp. strain showed specific activity of 25.97 U/mg; Km= 6.3 %; Vmax= 19.5 U/mg; pH optimum between 6.5 to 7.0 and temperature optimum from 25 °C to 35 °C. Tannase from Aspergillus niger strain presented enzymatic activity of 0.79 U/mL and tannase from Paecilomyces sp. strain reached 2.14 U/mg, pH and temperature optimum of 5.5 and 60 °C, respectively (AU) | |
| FAPESP's process: | 11/14840-5 - Screening newly microbial strains for tannase and lipase production |
| Grantee: | Érica Benjamim da Silva |
| Support Opportunities: | Scholarships in Brazil - Master |