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| Author(s): |
Giovanna Azevedo Celestrino
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) |
| Defense date: | 2020-12-04 |
| Examining board members: |
Maria da Gloria Sousa Stafocker;
Marcia de Souza Carvalho Melhem;
Suelen Andrea Rossi
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| Advisor: | Maria da Gloria Sousa Stafocker |
| Abstract | |
Dermatophytosis is a superficial fungal infection, characterized by the involvement of keratinized tissues, such as skin, hair and nails, but in some cases, can also become a deep infection. The main etiological agent is Trichophyton rubrum, accounting for approximately 80% of the cases. The role of phagocytes and pattern recognition receptors in fungal infections has been discussed in several studies; however, few studies address the role of monocytes and PRRs during the early stage of dermatophytosis. In this work, we analyzed the role of C-type lectin receptors and Toll like receptors expressed in human monocytes in the recognition of T. rubrum conidia. The results showed that phagocytosis of T. rubrum conidia by monocytes is associated with TLR2 or mannose receptor, but it was no altered by the blockade of mincle or dectin-1 receptors with their respective monoclonal antibodies. The fungicidal activity of monocytes was impaired in the absence of TLR2 or mannose receptor. Secretion of TNF-alpha by monocytes in the presence of T.rubrum conidia was reduced by the blockade of the receptors mincle, dectin-1, TLR2 or mannose. On the other hand, the IL1-beta secretion was impaired only by the blockade of TLR2 and mannose receptor. Secretion of IL-10 was not altered in the absence of signaling from any of the these receptors. Was demonstrated that TLR2 and mannose receptor expressed in human monocytes have a significant role in the recognition of T. rubrum conidia and in the production of inflammatory cytokines (AU) | |
| FAPESP's process: | 18/16146-8 - Chronic disseminated dermatophytosis due to Trichophyton rubrum: role of neutrophils in the effector mechanisms and in the modulation of the adaptive response of the human infection |
| Grantee: | Giovanna Azevedo Celestrino |
| Support Opportunities: | Scholarships in Brazil - Master |