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| Author(s): |
Juliana Mozer Sciani
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-07-31 |
| Examining board members: |
Daniel Carvalho Pimenta;
Ronaldo Zucatelli Mendonça;
Vitor Marcelo Silveira Bueno Brandão de Oliveira;
Ana Maria Moura da Silva;
Jose Roberto Machado Cunha da Silva
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| Advisor: | Daniel Carvalho Pimenta |
| Abstract | |
Echinometra lucunter, the sea urchin responsible for 50% of marine animals accidents, cause inflammation and pain by the spine penetration, effects attributed to the mechanical trauma. Accidents were reported after the ingestion of raw. The celomic fluid and spines were fractionated and purified, procedure repeated until pure molecules were obtained, tested for inflammation models. Histological analyses and enzymatic assays were performed. A molecule from spines and a peptide from the celomic fluid caused inflammatory effects. Moreover, a cathepsin B/X activity could be identified in the spines. An organized histological structure in the spine was observed, with cells embedded in a calcified matrix, as well as granulous cells displaying proteic contents, typical of secretory cells. It was possible to conclude that the spine and the celomic fluid of E. lucunter do contain inflammatory toxins that prolong the spine puncturing event itself, and the spine possesses a toxin secretory structure. The cathepsin would be present in a mechanism of tissue remodeling. (AU) | |
| FAPESP's process: | 07/08478-6 - Biological-driven biochemical characterization of peptides and proteins isolated from Echinometra lucunter. |
| Grantee: | Juliana Mozer Sciani |
| Support Opportunities: | Scholarships in Brazil - Doctorate |