Assessment of bioaccumulation and toxicity of innovative antifouling nanomaterials on tropical and neotropical marine invertebrates

• Grant number: 20/03004-0
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• Duration: April 01, 2021 - March 31, 2024
• Field of knowledge: Biological Sciences - Ecology - Applied Ecology
• Principal Investigator: Denis Moledo de Souza Abessa
• Grantee: Denis Moledo de Souza Abessa
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil
• Associated researchers: Cristiane Angélica Ottoni ; Fernando Cesar Perina ; Jeamylle Nilin Gonçalves ; Lucas Buruaem Moreira ; Paloma Kachel Gusso Choueri ; Renata de Britto Mari ; Rivelino Martins Cavalcante ; Roberto Carlos Domingues Martins ; Rodrigo Brasil Choueri ; Rubens Cesar Lopes Figueira ; Susana Patrícia Mendes Loureiro
• Associated scholarship(s): 22/01542-0 - Chemical characterization and bioaccumulation of innovative antifouling nanomaterials in neotropical and subtropical marine invertebrates, BP.TT

Abstract

After the banning of the tributyltin (TBT) as antifouling agent, many chemical compounds have been tested as active principle of anti-fouling coatings, such as the DCOIT, or Sea-Nine (4,5-dichloro-2-n-octyl-4-isothiazolin-3-one), which has been employed in the formulation of third generation anti-fouling paints. Nanomaterials also have been used as antimicrobial substances, especially silver nanoparticles (AgNP). Currently, new antifouling compounds have been developed, incorporating nanocapsules of silica impregnated with silver and containing the DCOIT, with the aim of reducing the environmental risks and increase the antifouling effectiveness. However, there are few studies reporting the ecotoxicological effects of those substances, isolated or combined, to non-target organisms as well as their ecological risks. Moreover, their respective mechanisms of action (at cellular and biochemical levels) are still not fully understood. This project aims to assess the toxicity of salts of Ag, the DCOIT, nanocapsules of silica (simple and impregnated with Ag) as well as the respective nanocapsules containing DCOIT, to marine invertebrates; this project also intends to investigate their respective mechanisms of action, in order to provide new information on the ecological risks related to these new biocides. To accomplish that, aqueous solutions and spiked sediments contaminated these substances will be evaluated by means of acute or chronic toxicity tests using the following organisms: Nitocra sp (Copepod), Tiburonella viscana (amphipod), Kalliapseudes schubartii (tanaid), Perna perna (bivalve) embryos, Echinometra lucunterm (sea-urchin), and Artemia sp (Anostraca). The mechanisms of action at the cellular and biochemical levels will be analyzed through biochemical and cellular biomarkers (GST, GSH, GPx, LPO, DNA damage, ATPases, Caspases and neutral red retention assay) and histopathological techniques, using adults of the bivalve Perna perna exposed to environmentally relevant concentrations of the respective compounds. The results will provide important information for the development of new antifouling paints, as well as on the effects and ecological risks caused by these new antifouling biocides, which will be helpful to reduce the environmental impacts related with these paints, and the establishment of environmental laws and enforcements. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CAMPOS, BRUNO GALVAO; DO PRADO E SILVA, MARIANA BRUNI MARQUES; AVELELAS, FRANCISCO; MAIA, FREDERICO; LOUREIRO, SUSANA; PERINA, FERNANDO; DE SOUZA ABESSA, DENIS MOLEDO; MARTINS, ROBERTO. Toxicity of innovative antifouling additives on an early life stage of the oyster Crassostrea gigas: short- and long-term exposure effects. Environmental Science and Pollution Research, JAN 2022. (20/03004-0, 19/19898-3, 18/25379-6, 17/10211-0, 19/24945-0)

DE CAMPOS, BRUNO GALVAO; FONTES, MAYANA KAROLINE; GUSSO-CHOUERI, PALOMA KACHEL; MARINSEK, GABRIELA PUSTIGLIONE; NOBRE, CAIO RODRIGUES; MORENO, BEATRIZ BARBOSA; LEMOS ABREU, FIAMMA EUGENIA; FILLMANN, GILBERTO; MARI, RENATA DE BRITTO; DE SOUZA ABESSA, DENIS MOLEDO. preliminary study on multi-level biomarkers response of the tropical oyster Crassostrea brasiliana to exposure to the antifouling biocide DCOI. Marine Pollution Bulletin, v. 174, JAN 2022. (17/10211-0, 17/04970-5, 20/03004-0)

MORAIS, LUCAS GONSALVES; GUSSO-CHOUERI, PALOMA KACHEL; ABREU, FIAMMA EUGENIA LEMOS; CASTRO, ITALO BRAGA; ABESSA, DENIS MOLEDO; CHOUERI, RODRIGO BRASIL. Multilevel assessment of chlorothalonil sediment toxicity to Latin American estuarine biota: Effects on biomarkers, reproduction and survival in different benthic organisms. Science of The Total Environment, v. 872, p. 11-pg., 2023-05-10. (20/03004-0)

CAMPOS, B. G.; MOREIRA, L. B.; PAULY, G. F. E.; CRUZ, A. C. F.; PERINA, F. C.; ABREU, F.; FILLMANN, G.; ABESSA, D. M. S.. Water and sediment toxicity and hazard assessment of DCOIT towards neotropical marine organisms. Environmental Pollution, v. 330, p. 9-pg., 2023-05-23. (16/02029-4, 21/06167-0, 20/00068-8, 20/03004-0, 21/08471-9, 18/23279-4, 17/10211-0)

SANTANA, LIGIA M. B. M.; DAMASCENO, EVILA PINHEIRO; LOUREIRO, SUSANA; SOARES, AMADEU M. V. M.; POUSAO-FERREIRA, PEDRO; ABESSA, DENIS M. S.; MARTINS, ROBERTO; PAVLAKI, MARIA D.. An Easy-to-Use Histological Technique for Small Biological Samples of Senegalese Sole Larvae. APPLIED SCIENCES-BASEL, v. 13, n. 4, p. 12-pg., 2023-02-01. (20/03004-0, 18/07098-0)

ABESSA, DENIS MOLEDO DE SOUZA; ALBANIT, LETICIA FRANCA; DE MOURA, PEDRO HENRIQUE PAIXAO; NOGUEIRA, VITORIA SOARES; SANTANA, FELIPE TEIXEIRA; FAGUNDES, KAINA; UEDA, MAYSA; MULLER, OTTO PATRAO DE OLIVEIRA; CESAR-RIBEIRO, CAIO. A Glow before Darkness: Toxicity of Glitter Particles to Marine Invertebrates. TOXICS, v. 11, n. 7, p. 13-pg., 2023-07-01. (20/03004-0, 18/25498-5, 22/01542-0, 22/09070-0, 20/09300-0, 22/06164-4)