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| Author(s): |
Rodrigo Fernando Gomes Olivindo
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2018-05-23 |
| Examining board members: |
Antônio Chaves de Assís Neto;
Cristina de Oliveira Massoco Salles Gomes;
Johanna Plendl
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| Advisor: | Antônio Chaves de Assís Neto |
| Abstract | |
The testes are the primary sex organs and stand out with two main functions: spermatogenesis and the production and secretion of hormones, among them testosterone, important hormone for spermatogenesis and sexual differentiation during embryonic development. The unfolding of the enzymatic chain involved with testicular steroidogenesis is directly or indirectly responsible for the production of androgens and may therefore be related as one of the possible etiologies for testicular neoplasms. In this study, the expression of the enzyme P450 aromatase ( P450arom) and serum testosterone control to establish the relationship between carcinogenesis and P450arom behavior in dogs with testicular neoplasms. Animals selected from castration campaigns were selected by the city of São Paulo, State of São Paulo, Brazil. Samples of testicular and blood tissues were collected from 6 adult dogs submitted to orchiectomies, of which two presented unilateral cryptorchidism with increase in testicular size, one without ectopic testicle, but volume was increased, and the others were used for elective study controls. The histopathological examination was the result of three neoplastic cases, one mixed tumor (seminoma intratubular and sertolioma) and the other two seminomas. Immunohistochemistry of testicular tissues showed positive immunolabeling for the P450arom enzyme in the Leydig and Sertoli cells and was later confirmed by western blotting in all the animals of the study, although the 3N animal (Neoplastic 3) presented a poorer immunolocalization in relation to the others. Free testosterone hormone dosage was performed through blood samples collected prior to surgical procedures. Oncologic patients presented values at the lower limit, but within the reference for the species, except 3N, which presented lower values when compared to 1N (Neoplastic 1) (101,90 ng / dL), 2N (Neoplastic 2) (158,40 ng / dL), 3N (95.8 ng / dL), 1C (Control 1) (42.9 ng / dL), 2C (41.5 ng / dL) and 3C (87.3 ng / dL). It is concluded that the P450 arom showed positive marking in dogs with testicular neoplasia, also confirmed in the quantifications by western blotting, which was expressed in all the animals of the study. (AU) | |
| FAPESP's process: | 16/20913-9 - Expression of steroidogenic enzymes (P450c17, 3BHSD, 17BHSD and P450 aromatase) and serum testosterone in control dogs with testicular neoplasms |
| Grantee: | Rodrigo Fernando Gomes Olivindo |
| Support Opportunities: | Scholarships in Brazil - Master |