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| Author(s): |
Natália Galdi Quel
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-02-05 |
| Examining board members: |
Tomomasa Yano;
Terezinha Knöbl;
Luciano Moura Martins
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| Advisor: | Tomomasa Yano |
| Abstract | |
E. coli isolated from cellulitis lesions in broiler chickens produce a citotoxin, called ECVF (Escherichia coli Vacuolating Factor), which causes intense cytoplasm vacuolization in avian cells, but not in mammalian cells. The importance of ECVF in the pathogenesis of avian cellulitis was assessed in this study. Purified ECVF was inoculated subcutaneously in broiler chickens, and induced signs of inflammation on subcutaneous, adipose and connective tissues. In citotoxicity assays, we verified that ECVF induced cytoplasmic and nuclear alterations, which can affect cellular metabolism directly, such as chromatin condensation and nuclear fragmentation, intense cytoplasm vacuolization, and disorganization of cytoskeleton, leading to apoptosis. It was also verified the interaction of ECVF with proteins of avian cells, instead of those from mammalian cells, suggesting the specificity of this toxin to this cells. Our results, supported by data from previous studies, suggest an important role of ECVF in the pathogenesis of avian cellulitis. (AU) | |
| FAPESP's process: | 11/03586-0 - Vat (vacuolating autotransporter toxin) produced by APEC: internalization, intracellular translocation and cytotoxic activity in avian cells |
| Grantee: | Natália Galdi Quel |
| Support Opportunities: | Scholarships in Brazil - Master |