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Viability of national and imported polyamide threads as an alternative to the modified TightRope technique for the treatment of spontaneous cranial cruciate ligament insufficiency in dogs

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Author(s):
Alois Foltran Müller
Total Authors: 1
Document type: Master's Dissertation
Press: Pirassununga.
Institution: Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT)
Defense date:
Examining board members:
Silvio Henrique de Freitas; Luís Gustavo Gosuen Gonçalves Dias; Eliana Cristina da Silva Rigo
Advisor: Silvio Henrique de Freitas
Abstract

The cranial cruciate ligament is one of the main responsible for the femorotibiopatellar joint stability. When ruptured or lacerated (insufficient), it provides instability of the tibia relative to the femur, leading to lameness and also to degenerative joint disease. The TightRope is an extracapsular stabilization technique in which a resistant ultrapolethylene tape, secured with endobuttons made of titanium, aims to prevent the cranial drawer movement and the tibial internal rotation. To check the polyamide (nylon) threads viability as an alternative to the ultrapolethylene tape, these were analyzed employing mechanical stress tests, indirect cytotoxicity assessment, scanning electron microscopy (SEM), and by infrared spectroscopy (IR). The tested threads were accommodated in a device, developed for this purpose, and steam-sterilized at 120°C for 15 minutes. The results revealed that the accommodation device created was efficient in maintaining the length and diameter of the steam-sterilized threads. Through the mechanical stress tests, it was possible to obtain the threads elastic limits and thus to relate its diameter to the weight of the dog with cranial cruciate ligament insufficiency (CCLI). The IR spectroscopy did not show polyamide molecular alteration. The indirect cytotoxicity test revealed that the imported polyamide is cytotoxic to the cell line tested and, therefore, its use is not recommended in vivo, despite the favorable mechanical test results. Thus, the national steam-sterilized nylon fishing line is a viable alternative for the CCLI treatment, has low cost, easy acquisition, and can be used in other types of articular surgeries and in veterinary fields that require a more resistant suture. (AU)

FAPESP's process: 18/02039-5 - Viability of national (Araty®-superflex) and imported (Otoni®-Platinum) polyamide yarns as an alternative to the modified TightRope technique in the treatment of spontaneous cranial cruciate ligament insufficiency in dogs
Grantee:Alois Foltran Müller
Support Opportunities: Scholarships in Brazil - Master