EVALUATION OF INTRA-ARTICULAR ADMINISTRATION OF ALLOGENIC MESENCHYMAL STEM CELLS SECRETOME IN DOGS UNDERGOING TIBIAL PLATEAU LEVELING OSTEOTOMY

• Grant number: 22/14243-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2023
• End date: February 28, 2025
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Bruno Watanabe Minto
• Grantee: Alefe Luiz Caliani Carrera
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The tibial plateau leaveling osteotomy (TPLO) is the main surgical technique to treat the cranial cruciate ligament disease (CCLD) in dogs, although osteoarthritis progression is not completely resolved, causing incomplete or suboptimal recovery in some patients. In this context, the adipose tissue-derived stem cells (ADSC) secretomes can perform like an adjuvant in joint recovery, because their potential regenerative and anti-inflammatory effects. The objective of this research is to evaluate the articular regenerative and anti-inflammatory effects of intra-articular and intraoperative administration of secretomes in patients submitted to TPLO. It will be evaluate 20 dogs with CCLD, undergoing TPLO, divided in two groups, the control will receive 0,7mL of placebo intra-articular, and the treated group will receive 0,7mL of solution containing 200-250µg of ADSC extracellular proteins, and these patients will be evaluate before and after surgery about function recovery, joint pain, articular radiography and ultrasonography, through the dosage of seric biomarkers IL-1², IL-8 and MCP-1 and by accelerometry, for peak positive acceleration and vertical load for tibia. It's expected to prove that the secretomes can improve the return to function in the treated group through the anti-inflammatory and articular regenerative effects, in addition to ensure the clinical viability to use these substances in dogs' surgical routine.