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| Author(s): |
Rodrigo Pimenta Carvalho
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2016-07-29 |
| Examining board members: |
Cassius Vinicius Stevani;
Etelvino Jose Henriques Bechara;
Luiz Francisco Monteiro Leite Ciscato;
Anderson Garbuglio de Oliveira;
Peter Wilhelm Tiedemann
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| Advisor: | Cassius Vinicius Stevani |
| Abstract | |
This thesis describes methods for purification and determination of molecular mass of fungal luciferin precursor from fruiting bodies of bioluminescent fungus Neonothopanus gardneri. The molecule it is the substrate of enzymatic reaction first step responsible for light emission in fungi, phenomenon known as bioluminescence. During PhD, it was optimized a qualitative assay in order to detect the luciferin precursor in solutions and also were developed methods for extraction and purification of the molecule of interest, always considering the low amount of molecule available in vivo and the susceptibility of molecule degradation when exposed to oxygen, light and extremes pH\'s. After establishing several chromatographic conditions for purification of luciferin precursor, it was possible to correlate a molecule with molecular mass of 246.05 u with a positive activity in the qualitative analytical assay. Later, a Russian group describe that the precursor of luciferin had molecular mass 246.05 u and molecular formula C13H10O5. Experiments were also performed in order to corroborate the molecular structure of the reaction product of light emission in fungi as well as determine its formation mechanism. (AU) | |
| FAPESP's process: | 12/05205-7 - Purification, characterization and mechanistic study with fungal luciferin |
| Grantee: | Rodrigo Pimenta Carvalho |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |