Advanced search
Start date
Betweenand

Mechanistic study of luciferin-luciferase interaction in fungi

Grant number: 18/06686-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Start date: August 01, 2018
End date: January 31, 2020
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal Investigator:Cassius Vinicius Stevani
Grantee:Marco Totini Tanezi
Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The present scientific initiation project aims to study the chemical mechanism of the reaction of fungal luciferin (3-hydroxyphispidine) with the recombinant luciferase of the fungus Neonothopanus nambi. Fungal luciferase, as a promiscuous enzyme, also accepts other substrates, leading to the emission of light. It is intended to perform kinetic studies with each of the thirteen luciferin derivatives available in the laboratory and to evaluate possible correlations between the observed light decay rate constant (kobs) with the oxidation potential of luciferins (Eox) and Hammett's Ã, for nine of the thirteen available derivatives. Fluorescence quantum yield values (¦FL) will also be determined for natural and synthetic oxyluciferin. Finally, free-energy relationships will be investigated between lnkobs vs. Eox, lnkobs vs. Ã and ln¦FL vs. the polarity of the solvent. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)