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Study of the variations of the mosquitoes assemblages (Diptera: Culicidae) in different strata of the vegetation and its relation with eating habits

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Author(s):
Eduardo Evangelista
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT)
Defense date:
Examining board members:
Mauro Toledo Marrelli; Tamara Nunes de Lima Camara; Regiane Maria Tironi de Menezes; Delsio Natal
Advisor: Mauro Toledo Marrelli
Abstract

An entomological study was carried out in the extreme limits of the municipality of São Paulo, which have significant vegetation cover in the form of Conservation Units, represented in the north, by the State Park of the Cantareira (PEC), where epizootics and cases human of Yellow Fever have been occurred in the last years, and in the South by the Environmentally Protected Area Capivari Monos, where asymptomatic cases of malaria-bromelia have been occurred. Mosquitoes were collected from February 2015 to April 2017 in seven environments with peri-urban, intermediate and wild features. CDC Traps were installed in the canopy and at ground level, with the objective of verifying the vertical stratification of mosquito species. Aspiration and Shannon traps complemented the collection of engorged females to verify the feeding behavior by PCR technique. Nine mitochondrial cytochrome B oligonucleotides were used to this propose. Richness, abundance, composition and equability were used to verify the diversity of environments. In total, 10,070 adult culicidis were collected, comprising 95 species / taxonomic categories distributed in sixteen genera. At APA, there were 75 species / taxonomic species and 7,472 specimens and a total of 67% were more frequent in the canopy. The species Culex (Culex) nigripalpus and Anopheles (Kerteszia) cruzii, occurred more frequently and canopy stratum in all the environments surveyed. In the PEC, 58 species / taxonomic categories and 2,598 specimes were collected and 55% were collected in the ground strata. Wyeomyia (Prl.) confusa (ground), Culex (Mel.) vaxus (canopy) and Limatus durhamii (ground) were more abundant. Regarding the feeding habits, 386 samples were obtained, Anopheles cruzii represented 70% (270), feeding mainly on human host in the ground strata. The presence of vector culicids evidences the high epidemiological importance of the studied environments, deserving attention of the Public Health Authorities due to the presence and interaction of mosquito species and their sources of blood feeding. (AU)

FAPESP's process: 15/25820-6 - Study of changes of assemblages mosquitoes (Diptera: Culicidae) in different strata of vegetation and its relationship with eating habits
Grantee:Eduardo Evangelista
Support Opportunities: Scholarships in Brazil - Master