Biodiversity of yeasts from marine and terrestrial antarctic ecosystems and prospection of lipases.

Author(s)

Alysson Wagner Fernandes Duarte

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-03-06

Examining board members

Lara Durães Sette; Welington Luiz de Araujo; Eleonora Cano Carmona; Itamar Soares de Melo; Fernando Carlos Pagnocca

• Advisor: Lara Durães Sette

Abstract

The Antarctic continent is characterized by restrictive environmental conditions of climate, habitats and biogeography. In this sense, the objective of this study was to evaluate the diversity of yeasts in marine and terrestrial samples of the Antarctic environments, as well as the propspecting of lipases that can act in proceses that require low and moderate temperatures. According to the results, 97 yeasts were recovered from different samples collected in OPERANTAR XXVIII (summer 2010), representing 21 different species. Among them, 45 showed lipase activity and two were selected for the optimization of enzyme production: C. laurentii L59 and L. scotti L117. Extraction of L. scottii L117 lipase was assessed by Aqueous Two-Phase System and the micellar system TX-114 was the most efficient for partial enzyme purification. Finally, the diversity evaluation of 405 isolates from seaweeds and lichens (collected in XXXII OPERANTAR, summer of 2013) revealed the occurrence of 24 taxa from macroalgae samples and 18 taxa from lichens samples, being only five substrates common to these two substrata. (AU)

• FAPESP's process: 10/08352-5 - Biodiversity of marine and terrestrial yeasts from Antarctic ecossystems and prospection of lipases
• Grantee: Alysson Wagner Fernandes Duarte
• Support Opportunities: Scholarships in Brazil - Doctorate