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| Author(s): |
Hebert Fabricio Culler
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-04-20 |
| Examining board members: |
Marcia Regina Franzolin;
Rene Peter Schneider;
Mônica Aparecida Midolli Vieira
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| Advisor: | Marcia Regina Franzolin; Marcelo Palma Sircili |
| Abstract | |
The atypical Escherichia coli enteropathogenic (EPECa) are capable to cause the lesion A/E and they don\'t transport the pEAF. Microbial biofilms are defined as complex communities formed by microrganisms adhered to surfaces embedded in an exopolysacharidic matrix. The objective of this study was to verify the capacity of formation of biofilm of 92 strains of atypical EPEC in abiotics and pre-fixed cells surfaces, using three methodologies (counting of UFC/cm2), colorimetric assay with violet crystal and CLSM). The gene shf and bfpA seem not to have relationship with the biofilm formation. There was not significant difference of biofilm formation in the tested surfaces. Through CLSM it was possible to verify the biofilm formation in EPECa. The biofilms visualized in CLSM revealed a great heterogeneity of the strains. A qualitative methodology, like CLSM should be used to indicate the biofilm formation. The adhesion and biofilm formation for atypical EPEC of long period can be a possible explanation for the cases of persistent diarrhea. (AU) | |
| FAPESP's process: | 07/06104-1 - Biofilm in atypical E.coli |
| Grantee: | Hebert Fabricio Culler |
| Support Opportunities: | Scholarships in Brazil - Master |