In vitro effects of fluoride in the anticarie potential of CO2 laser on demineralized dental enamel

Author(s)

Bruna Raquel Zancopé

• Document type: Master's Dissertation
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2012-02-29

Examining board members

Marinês Nobre dos Santos; Karine Laura Cortellazzi; Patricia Moreira de Freitas

• Advisor: Marinês Nobre dos Santos

Abstract

The aim of this study was to investigate if CO2 laser irradiation enhances fluoride uptake by demineralized dental enamel and to evaluate whether enamel lesion progression can be inhibited by CO2 laser (?=10.6 ?m) irradiation with two fluencies (11.3 J/cm² and 20.0 J/cm²) combined with acidulated phosphate fluoride (APF) application. 315 specimens of carious enamel (SCE) were used in two experiments. In experiment 1, 150 SCE were allocated to 10 groups, which were irradiated combined with one APF application performed either before, during or after irradiation. Following, 50 specimens had their surface examined for morphological changes by scanning electron microscopy (SEM). Fluoride as CaF2 formed on enamel surface was determined in 100 SCE (n=10, 10 groups). After CaF2 analysis, a new SEM analysis was performed in 50 specimens. In experiment 2, 165 SCE (11 groups, n=15) were submitted to the same treatments as in experiment 1 + a caries lesion only group. After pH cycling, fluoride in demineralizing (Demin) and remineralizing (Remin) solutions, microhardness and lesion depth were determined. The results were analyzed by ANOVA and Tukey test and by Kruskal-Wallis rank test and Student-Newman-Keuls test ('alpha'=0.05). The results showed that irradiation alone or combined with APF enhanced CaF2 formation on enamel surface, fluoride concentration in de-remin solutions and inhibited lesion progression (p?0.05). SEM observations showed evidences of melting, fusion and cracks. In conclusion, laser irradiation alone or combined with APF enhances CaF2 uptake and inhibits lesion progression on demineralized dental enamel. However no synergic effect was found (AU)

• FAPESP's process: 09/13295-3 - EVALUATION OF THE STRONG AND WEAKLY ON EFFECT OF THE FLUORID IN THE EFFECTIVENESS OF THE CO2 LASER IN REDUCING THE DEMINERALIZATION AND INCREASING THE REMINERALIZATION OF THE DENTAL ENAMEL - STUDIES IN VITRO
• Grantee: Bruna Raquel Zancopé
• Support Opportunities: Scholarships in Brazil - Master