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| Author(s): |
Vânia Aparecida Blasques Bueno
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2010-07-01 |
| Examining board members: |
Luiz Henrique Catalani;
Guilhermino José Macedo Fechine;
Edvani Curti Muniz;
Mario Jose Politi
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| Advisor: | Luiz Henrique Catalani |
| Abstract | |
Micro and nanostructured hydrogels (three-dimensional polymeric networks capable of absorbing large amounts of water) have great potential as biomaterials in applications such as drug delivery devices and embolic agents. In this work techniques to produce micro and nanostructured hydrogels from poly(N-vinyl-2-pyrrolidone) (PVP) were studied from various crosslinking reactions like Fenton, photo-Fenton and direct photolysis. PVP microgels were obtained from crosslinking of polymer spheres obtained by electrospraying a polymer solution or from droplets of aqueous polymer solution in w/o emulsion. In the first case, particles were obtained that, when crosslinked with the Fenton reaction, showed undefined morphology. In the second case, spherical PVP hydrogel microparticles were obtained, which were formed by crosslinking with Fenton reaction, resulting in smaller particles - about 1µm - while with photo-Fenton reaction it generated larger particles - about 34 µm. Sub-micrometric hydrogels were obtained from lecithin vesicles as formatting system through crosslinking with photo-Fenton reaction. These vesicles were able to encapsulate large amounts of polymer, in spite of its high average molecular weight. Hydrogels synthesized by this method are formless, with narrow diameter distribution and low swelling properties. Finally, nanogels (hydrogel nanoparticles) were obtained from crosslinking of PVP by the Fenton reaction (Fe2+ in presence of H2O2), using aqueous pool of CTAB reverse micelles as formatting system. The thus formed nanogels are spherical and present interesting properties such as swelling ratio of 6000, a level unreported in literature, which classifies them as superabsorbent hydrogels. Moreover, the swelling is susceptible to control either by the removal of complexed Fe3+ ions or by acidity and ionic composition of the medium. During the nanogels synthesis development, the influence of Fe3+ in the arrangement of the polymer solution and of the hydrogel was studied. Morever, the polymer crosslinking kinetics inside the micelles and a comparison between two different micellar systems in formatting of nanogel were analyzed. The product analysis also revealed low toxicity of the obtained material by in vitro experiments using macrophages and fibroblasts. (AU) | |
| FAPESP's process: | 06/01458-7 - Production of nano- and microstructured hydrogels |
| Grantee: | Vânia Aparecida Blasques Bueno Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |