In vivo characterization of murine mesenchymal stem cells from bone marrow and compact bone in relation to its heterotopic hematopoietic niche generation potential

Author(s)

Rafaela Rossetti

• Document type: Master's Dissertation
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC)
• Defense date: 2020-03-20

Examining board members

Geraldo Aleixo da Silva Passos Junior; Carolina Caliári Oliveira

• Advisor: Dimas Tadeu Covas

Abstract

The hematopoietic stem cell niche is the microenvironment composed of different types of cells and stimulus that regulate self-renewing, differentiation and proliferation of the hematopoietic stem cells. The mesenchymal stem cells (MSCs) are important components of the hematopoietic niche. The MSCs were previously studied from bone marrow, it\'s known that, in culture, they are able to form colonies and differentiate giving rise to cells of adipose, bone and cartilaginous tissue, as well as originate heterotopic hematopoietic niche when implanted. These cells were initially described with perivascular location in the bone marrow, although later studies have shown that the MSCs are also present in the endosteum region. So, it\'s been suggested the hypothesis of existing two distinct populations of MSCs in the bone marrow, one located in the endosteum region, being mainly responsible for generating bone tissues during the development phase and for tissue homeostasis, and another, of perivascular location, which would act in tissue repair during adulthood. Therefore, in this work, MSCs isolated from bone marrow (BM-MSCs) and compact bone (CBMSCs) from C57BL/6gfp mice were compared to their in vitro characteristics and their capacity to originate a heterotopic hematopoietic niche. It\'s been observed that the MSCs have shown differences related to their frequency of CFU-F, immunophenotypic profile, capacity of differentiation and gene expression. Additionally, the evaluation of three different protocols to standardize MSCs implantation, aiming the hematopoietic niche formation, has shown that implantation of Matrigel along with hydroxyapatite granules, endosteum cells and BM-MSCs were able to provide the generation of a heterotopic hematopoietic niche. (AU)

• FAPESP's process: 17/12663-5 - In vivo characterization of murine mesenchymal stem cells from bone marrow and compact bone in relation to its heterotopic hematopoietic niche generation potential
• Grantee: Rafaela Rossetti
• Support Opportunities: Scholarships in Brazil - Master