Full text
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| Author(s): |
Eduardo Marcos Balciunas
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2013-09-13 |
| Examining board members: |
Ricardo Pinheiro de Souza Oliveira;
Attilio Converti;
Michele Vitolo
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| Advisor: | Ricardo Pinheiro de Souza Oliveira |
| Abstract | |
The objective was the production of bacteriocins by Bifidobacterium animalis subsp. lactis, comparing the synthetic culture medium BSM (Bifidus Selective Medium) and MRS (Man Rogosa and Sharpe) with the natural culture medium (whey). Initially, growth and post-acidification curves were determined, consumption of glucose, lactose and B. lactis bacteriocin production by fermentation processes using culture media BSM, MRS and milk whey (SL).The indicator organisms used in the test sensitivity to bacteriocin produced by B. lactis were Lactobacillus sakei, Escherichia coli and Listeria monocytogenes. Given the strain B. lactis one aerotolerant species of bacteria, it was conducted in culture medium BSM, a preliminary study assessing the growth, by varying the agitation (25, 50, and 100 rpm) with cultivation time of 30h at 37°C temperature. The best results of cell growth (9.4 log CFU / mL) were obtained at 50 rpm agitation. After the best condition of agitation (50 rpm) and temperature (37°C) determination, it was performed on whey, a study of growth, acidification and consumption of lactose, varying the concentration of total dissolved solids (5, 10, 15, 20 and 25% w/v), to settle the best concentration of whey for studies of supplementation. The highest amount of biomass produced, combined with the lowest post acidification was found in whey at 10% (w/v) of total solids, wherein the microorganism presented at the end of culture (30 hours) a counting of 9.13 log CFU/mL and pH 4.29, respectively. It was also verified the influence from the culture media on B. lactis growth and production of bacteriocin on a rotary shaker (shaker), which was the comparative analysis from the effect of supplementation by 1% of the following ingredients: yeast extract (EL), inulin (I), L-cysteine (IC) and Tween 80 (T80). The best growing conditions found for higher biomass and bacteriocin production were obtained from the whey concentration of 10% (w/v) supplemented with 1% yeast extract (9.9 log CFU/ml to 200 AU/mL). In the final stage of the work, these conditions were tested in bench fermentor, where it was observed that the growth of Bifidobacterium lactis was 10% higher than in the rotary shaker. Regarding the activity of bacteriocin produced in fermenter bench, there was no difference in the rotary shaker (200 AU / mL). This difference in growth may be due to the better anaerobic conditions offered in bench fermentor, which was the injection of nitrogen into the medium, and in a rotary shaker, the anaerobic condition was generated by an external agent to the medium (use of anaerobic jars). Through this study, it can be concluded that bacteriocin production by B. lactis is achievable and shows promising results when used the combination whey added yeast extract, which showed antimicrobial activity against the strain Listeria monocytogenes. The optimization process bench fermentor has been shown interesting as bacteriocin production at industrial level. (AU) | |
| FAPESP's process: | 11/14048-0 - Production of bacteriocin by Bifidobacterium lactis using milk whey as a substrate |
| Grantee: | Eduardo Marcos Balciunas |
| Support Opportunities: | Scholarships in Brazil - Master |