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| Author(s): |
Fabricio Edgar de Moraes
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Matemática e Estatística (IME/SBI) |
| Defense date: | 2016-07-15 |
| Examining board members: |
Carlos Alberto Labate;
Helaine Carrer;
Paulo Cavalcanti Gomes Ferreira;
João Carlos Setubal;
Guilherme Targino Valente
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| Advisor: | Carlos Alberto Labate |
| Abstract | |
Sugarcane (Saccharum spp.) is one of the most important cultivated grasses of the world and Brazil is the largest producer, it has become an important crop due to high carbon assimilation rates allowing the synthesis and accumulation of large amounts of sucrose in their internodes. Thus, it is necessary a high understanding of the molecular mechanisms involved in the regulation of sucrose accumulation in this plant. These mechanisms have been studied at various levels, such as gene identification and localization, identification of quantitative trait locus controlling, transcriptome, proteome, characterization and metabolites identification. With all these studies is evident the necessity for a holistic approach to global understanding of the plant during the sucrose accumulation. Thus, this work aims to integrate metabolomics and proteomics data from tissues of sugarcane variety SP80-3280, during plant development and sucrose accumulation, using bioinformatics to link these results by regularized canonical correlation analysis in a systems biology approach. The results indicate differences in the metabolic and protein profile of sugarcane during development and sucrose accumulation. Metabolites classes have been proposed that may be related to sugarcane sucrose accumulation as glycerolipids, glycerophospholipids, coumarins and derivatives, steroids and steroid derivatives and fatty acyl. In addition, some proteins have been proposed that may be related to sucrose accumulation, where the most highlighted were the histones. In the biological correlations networks, have been also observed correlations between possible metabolites and proteins that can be correlated with the accumulation of sucrose in sugarcane (AU) | |
| FAPESP's process: | 12/12521-2 - Bioinformatics applied to systems biology for regulatory factors identification of the sucrose accumulation in sugarcane stalk |
| Grantee: | Fabrício Edgar de Moraes |
| Support Opportunities: | Scholarships in Brazil - Doctorate |