Low intensity LASER therapy in the control of chronic neuropatic pain and the regeneration of the ischial nerve of rats.

Author(s)

Mara Evany de Oliveira Silva

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2019-03-12

Examining board members

Marucia Chacur; Raquel Chacon Ruiz Martinez; Raquel Simoni Pires; Sandra Coccuzzo Sampaio Vessoni

• Advisor: Marucia Chacur

Abstract

The laser therapy technique is a noninvasive method used by several health areas, and has been shown to be effective in treat pain sensitivity. The present study aims to elucidate the effects of this therapy on molecular changes induced by chronic constriction (CCI) in rats. This technique was applied every other day, during 10 sessions. At the end of treatment, the animals were euthanized and the posterior root ganglia (DRG L4-L6) and sciatic nerve were removed for further analysis. The DRG\'s were processed for the detection of Substance P (SP) and transient potential receptor type I (TRPV1). In addition, we submit the sciatic nerve for the detection of neural growth factor (NGF) and zero protein (P0). Our results demonstrated a reversal of hypernociception in animals treated with LASER. Furthermore, we observed an increase in SP and TRPV1 expression in animals with lesion and a decrease after LASER treatment. With respect to the tests referring to the sciatic nerve, we could observe that there was an increase in the optical density for NGF and P0 after treatment. These results can be observed qualitatively by fluorescence immunohistochemistry. Our results together demonstrate the efficacy of laser therapy for the reversal of hypernociception of animals with lesions. Furthermore, this technique is able to modulate the release of mediators that participate in the algic process; in addition, this technique is also able to induce regeneration of sciatic nerve in rats. (AU)

• FAPESP's process: 14/24660-2 - Low level laser therapy in the management of chronic neuropathic pain in sciatic nerve regeneration in rats
• Grantee: Mara Evany de Oliveira Silva
• Support Opportunities: Scholarships in Brazil - Doctorate