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| Author(s): |
Fernanda Piccolo Pieruzzi
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-12-16 |
| Examining board members: |
Eny Iochevet Segal Floh;
Claudio José Barbedo;
Marcos Silveira Buckeridge;
Massuo Jorge Kato;
André Luis Wendt dos Santos
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| Advisor: | Eny Iochevet Segal Floh |
| Abstract | |
The aim of this work was the study of cryopreservation A. angustifolia plant systems. A protocol for cryopreservation of A. angustifolia cell cultures was developed based on optimum DMSO concentration. It was observed that cells exposed to cryoprotectants showed lower levels of PAs, ABA and ROS compared to cells that were not exposed to them. Two low molecular weight bands could be observed more intensely stained only in the cells that were exposed to the cryoprotectant. The zygotic embryos were used as a model to study different aspects of the biophysical process of cryopreservation such as the permeability of cryoprotectants, osmotic and response characteristics of water freezing in tissue. The results of this work led to a better understanding of different physiological, biochemical and biophysical factors during cryopreservation in recalcitrant species and open perspective to the establishment of the ex situ conservation of A. angustifolia. (AU) | |