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| Author(s): |
Fábio Borgatto
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2000-11-28 |
| Advisor: | Murilo de Melo |
| Abstract | |
This work was aimed at investigating the effect of Chrysanthemum morifolium Ramat plants mineral nutrition on tissue explants callogênesis induction and callus growth. Chrysanthemum morifolium Ramat cv "Bi Time" plants were sandy grown in plastic pots mantained in greenhouse under 8/16 hour photoperiod and environmental temperature. These plants were treated with mineral nutritive solution containing differents leveIs of nitrogen, phosphorus, potassium, calcium and magnesium for 60 days. ExpIants (leaves and shoots) were collected after 30, 45 and 60 days after mineral treatment started and inoculated on Murashige and Skoog growth medium suplemented with kinetin 0.1 mg.L-1 and naphthalen acetic acid 5.0 mg.L-1. lncubation conditions were 8/16 hours photoperiod and 26+ 2° C temperature. Evaluations based on callus fresh weight were carried out at 30 days after explants inoculation. The obtained results indicated the callogenesis induction and the callus cells growth to be nitrogen and potassium leveIs and treatment duration dependent. LeveIs of Phosphorus higher or Iower than that of complet nutritive solution caused reduced calogenese and callus growth. Callogenesis induction was great by calcium dependent, being inversely proportional to the calcium concentration e directly proportional to the treatment duration. Magnesium treatment affects callogenesis and cell growth positively,but the long duration calcium treatments reduced callogenesis on shoot explants (AU) | |