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| Author(s): |
Bruna Pereira de Morais
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-10-31 |
| Examining board members: |
Irina Kerkis;
Niels Olsen Saraiva Câmara;
Lygia da Veiga Pereira Carramaschi
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| Advisor: | Irina Kerkis |
| Abstract | |
Retina is composed of specialized neuronal cells, which in case of damage are unable to regenerate. CTIPD is a promissory source to replace the injured tissue because they can differentiate into almost all cell types. The aim of the study was to evaluate the differentiation potential of CTIPD in retinal neurons. CTIPD were submitted to neurosphere formation and evaluated by the expression of nestin, <font face=\"Symbol\">b-III-tubulin and Pax-6. From the neurosphere was performed the induction of differentiation into retinal neurons, which was assessed by the expression of specific markers of retinal differentiation. We observed a positive reaction with nestin, <font face=\"Symbol\">b-III-tubulin and Pax-6 in the sphere, suggesting a commitment to the neural/retinal lineage. In differentiated cells we found a higher expression of Chx-10 and Crx and a lower expression of Nrl, Calbindin, Recoverin and Rhodopsin. This study suggests that CTIPD show potential development into retinal neurons. However, additional studies are needed to elucidate the formation of the neural retina. (AU) | |
| FAPESP's process: | 10/14385-3 - Study of in vitro differentiation of human immature dental pulp stem cells into retinal neurons-like cells |
| Grantee: | Bruna Pereira de Morais |
| Support Opportunities: | Scholarships in Brazil - Master |