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| Author(s): |
Sandra Fernanda Suárez Patiño
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2016-05-06 |
| Examining board members: |
Soraia Attie Calil Jorge;
Adriano Rodrigues Azzoni;
Marcos Alexandre Nobre Lemos;
Ronaldo Zucatelli Mendonça;
Carlos Roberto Jorge Soares
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| Advisor: | Soraia Attie Calil Jorge |
| Abstract | |
Eukaryotic cells are cultured with serum, however current biotechnological approaches of cell culture need to avoid using of this supplement, due to the high costs, lot-to-lot variation and risk of contamination. Thus, our aim was to express the recombinant protein: GFP (green fluorescent protein); NS3 (Hepatitis C virus non-structural protein 3) and RVGP (rabies virus glycoprotein) in BHK-21 cells cultured in serum free culture based on Semliki Forest Virus system. The results of this work showed that cells cultured in serum-free media (SFM) were grown efficiently, they were produce more recombinant viral particles when compared with cells supplemented with SFB. These viral particles can be used directly for immunization, since generated amplification and expression efficient of different proteins within the host cell. (AU) | |
| FAPESP's process: | 12/00978-8 - PRODUCTION OF RECOMBINANT PROTEINS IN BHK-21 CELLS CULTURED IN SERUM-FREE MEDIUM |
| Grantee: | Sandra Fernanda Suárez Patiño |
| Support Opportunities: | Scholarships in Brazil - Doctorate |