Concentration of bioactive compounds analysis and evaluation of acute toxicity in vivo of the diterpenes cafestol and kahweol from green beans coffee oil obtained by supercritical and pressurized fluid extractions

Author(s)

Naila Albertina de Oliveira

• Document type: Master's Dissertation
• Press: Pirassununga.
• Institution: Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT)
• Defense date: 2015-07-22

Examining board members

Alessandra Lopes de Oliveira; Cintia Bernardo Gonçalves; Frederico Augusto Mazzocca Lopes Rodrigues

• Advisor: Alessandra Lopes de Oliveira

Abstract

This study aimed to evaluate the bioactivity of the diterpenes in green coffee oil, cafestol (C), and kahweol (K) using supercritical fluid extraction (SFE- CO2), and to optimize the extraction with pressurized liquid extraction (PLE) in batch by using a rotatable central composite design (RCCD2). This issue has innovative and novel character, since the pressurized liquid extraction technology available until now for analyte extraction has been studied in vegetable oil extraction processes such as Pequi oil, green coffee oil, and Pitanga seeds extracts, at LTAPPN. This technique uses organic solvents and high extraction temperature, which increases the solvent solubilization capacity. The use of high pressure accelerates the diffusion rates into the pores of the matrix, due to the lower solvent viscosity. This behavior provides greater penetration of the solvent into the matrix, increasing solvent extraction capacity. The supercritical fluid extraction (SFE) is a clean technology as it does not employ organic solvents, besides being a promising alternative to obtain extracts enriched with bioactive compounds. The results of the acute activity of diterpenes in green coffee oil extracted by SFE and PLE showed that although no lethality was observed in the animals using the oil extracted by SFE at a dose of 2,000 mg / kg in an acute toxicity study, and 25, 50, and 75 mg / kg in a repeated dose toxicity study, changes were observed in biochemical, hematological, and histological parameters. However, more in vivo studies about the effects of green coffee oil are required for pharmaceutical, food, and cosmetic applications. Similarly, the technological innovation to obtain the green coffee oil aims at obtaining extracts enriched in diterpenes, preventing degradation and increasing stability. The results indicate that green coffee oil extracted by SFE and PLE (in batch) have high concentrations of the active compounds cafestol and kahweol, and the best extraction condition was the trial 4 (70 º C, and 8 min), and the highest oil yield was 9.78%. (AU)

• FAPESP's process: 13/03371-0 - CONCENTRATION OF BIOACTIVE COMPOUNDS ANALYSIS AND EVALUATION OF ACUTE TOXICITY IN VIVO OF THE DITERPENES CAFESTOL AND KAHWEOL FROM GREEN BEANS COFFEE OIL OBTAINED BY SUPERCRITICAL AND PRESSURIZED FLUID EXTRACTIONS.
• Grantee: Naila Albertina de Oliveira
• Support Opportunities: Scholarships in Brazil - Master