Full text
| |
| Author(s): |
Vanessa Gomes Batista
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-07-17 |
| Examining board members: |
Gil Benard;
Sandro Rogerio de Almeida;
Magnus Ake Gidlund;
Alexandre de Castro Keller;
Anita Hilda Straus Takahashi
|
| Advisor: | Gil Benard |
| Abstract | |
Glycosphingolipids (GSL) may insert into specific membrane regions (lipid rafts) when added to cell culture, modulating its function. We evaluated whether glucosylceramide (CMH) and glycoinositol-phosphoceramide (GIPC), extracted from Paracoccidioides brasiliensis, are able to modulate immune cells functions of cured paracoccidioidomycosis (PCM) patients (control group) or individuals who never had PCM (cured group). CMH increased CD80 and CD86 expression on monocytes, increased lymph-proliferation on PBMCs and reduced lympho-proliferation in co-cultures with dendritic cells (DCs). In cured groups, CMH decreased gp43 and CMA- induced lymph proliferation, reduced IL-10 and increased IL-12 production by DCs. GIPC increased phagocytic capacity, reduced the maturation levels on DCs and DC capacity to induce lymph-proliferation. iNKT cells were analyzed and there were no differences in iNKT cells number, expansion capacity and cytokines production among the groups. In conclusion, GSL extracted from P. brasiliensis are able to modulate immune cells functions. (AU) | |