Development of a chemically defined medium for capsular polysaccharide production by Streptococcus pneumoniae serotype 14.

Author(s)

Anne Letícia Silva Ferri

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-06-14

Examining board members

Viviane Maimoni Gonçalves; José Gregorio Cabrera Gomez; Adriana Celia Lucarini

• Advisor: Viviane Maimoni Gonçalves

Abstract

In this work we assessed the influence of different carbon sources (CS) and defined medium compositions on cell growth and polysaccharide PS14 production. In bath, glucose, sucrose and fructose were tested at different concentrations. Also, media were tested with absence of the amino acids: asparagine, aspartic acid, phenylalanine, serine, alanine, threonine, tryptophan, lysine, and tyrosine, and vitamins/cofactors: folic acid, pyridoxamine, p-aminobenzoic acid, riboflavin and <font face=\"Symbol\">b-NAD, besides the addition of higher concentration of amino acids identified as important. In continuous cultivation, dilution rates (D) from 0.1 h-1 to 0.5 h-1 were evaluated as well as the influence of nitrogenous bases. The medium containing sucrose as CS, absence of amino acids and vitamins and addition of twice glycine isoleucine, leucine, valine, and triple glutamine led to higher production of PS14 (441mg/L). D of 0.4 h-1 showed higher productivity and adenine in standard concentration produced greater amounts of PS14. (AU)

• FAPESP's process: 11/02805-0 - Determination of essential amino acids and influence of nitrogenous bases on the continuous cultivation of Streptococcus pneumoniae serotype 14
• Grantee: Anne Leticia Silva Ferri
• Support Opportunities: Scholarships in Brazil - Master