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Taxonomy and molecular phylogeny of Myxozoa parasites of freshwater fish from natural environment and fish farm

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Author(s):
Mateus Maldonado Carriero
Total Authors: 1
Document type: Master's Dissertation
Press: Pirassununga.
Institution: Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT)
Defense date:
Examining board members:
Antônio Augusto Mendes Maia; Anderson Luis Alves; Maria José Tavares Ranzani de Paiva
Advisor: Antônio Augusto Mendes Maia
Abstract

The phylum Myxozoa has a great diversity, with about 2300 known species, which infect mainly fishes, but also amphibians, reptiles and birds. In this study, freshwater fishes were caught in the Brazilian Pantanal wetland (Mato Grosso and Mato Grosso do Sul states) and in the Mogi Guaçu River and CEPTA/ICMBio\'s fishfarm (São Paulo state) aiming the molecular and morphological studies of myxosporeans parasites of 6 fish species. The results of molecular (amplification and sequencing of the 18S rDNA gene) and morphological analysis revealed the occurrence of 11 species of myxosporeans, five of them infecting both Pseudoplatystoma corruscans and Pseudoplatystoma fasciatum, three parasites of Salminus brasiliensis, one specie infecting Brycon hilarii, one infecting Zungaro jahu and another infecting Piaractus mesopotamicus. Of these eleven species, five are not yet described by literature. The phylogenetic analysis, using the Neighbor-joining method, showed that the species clustered mainly according to the phylogenetic distance of their hosts and that all species of South America were grouped in a monophyletic clade. In some positions of the phylogenetic tree was observed that tissue tropism and/or organ of infection characterized an important factor in evolutionary selection. Less frequently was also observed some groups containing species which the major apparent relation is its geographical position, however, new studies are still needed to determine the true role of this factor in the evolution of myxosporeans. (AU)

FAPESP's process: 09/03320-0 - Taxonomy and molecular phylogeny of mixosporeans parasites of dourado (Salminus brasiliensis), pintado (Pseudoplatystoma corruscans) and cachara (Pseudoplatustoma fasciatum) from natural environment and fish farms
Grantee:Mateus Maldonado Carriero
Support Opportunities: Scholarships in Brazil - Master