Study about the reduction of oxidative stress in equine semen from the addition of quercetin on cooling and freezing extenders

Author(s)

Luis Fernando Mercês Chaves Silva

• Document type: Master's Dissertation
• Press: Botucatu. 2016-06-06.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
• Defense date: 2016-04-27
• Advisor: Frederico Ozanam Papa

Abstract

The emergence of biotechnologies applied to the equine semen as cooling and freezing process represented a major breakthrough for Equine, especially for allowing a better use of the ejaculate, optimizing the use of genetically superior animals. However the application of these techniques induces thermal stress on sperm cells, resulting in physical and chemical injuries, among them a significant rise in the production of reactive oxygen species (ROS). These unstable agents when in excess, are able to react and promote damage to enzymes, lipid membrane and nucleic acids, thereby reducing the viability of cryopreserved sperm. Thus is required to introduce elements that perform antioxidant function in cooling and freezing extenders. The quercertina, a polyphenol of the flavonoids group, is considered a potent antioxidant by reducing the emergence of the main ROS, chelate metal ions producers of free radicals and stabilize the lipid peroxidation. Despite this, the efficiency of application of this antioxidant on the equine ejaculate during the process of cryopreservation is still controversial and poorly referenced, requiring further studies in order to determine if there is influence of their use in the ejaculate of stallions during cooling and freezing. (AU)

• FAPESP's process: 14/01681-4 - Study about the reduction of oxidative stress in equine semen from the addition of quercetin on freezing and cooling extenders
• Grantee: Luis Fernando Mercês Chaves Silva
• Support Opportunities: Scholarships in Brazil - Master