Phylogeny and taxonomy of myxozoans parasites of fish from inland rivers of the State of São Paulo

Author(s)

Diego Henrique Mirandola Dias Vieira

• Document type: Doctoral Thesis
• Press: Botucatu. 2020-03-11.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu
• Defense date: 2020-02-18
• Advisor: Rodney Kozlowiski de Azevedo

Abstract

Parasites of aquatic organisms present considerable relevance to study because of their impact on aquaculture and fisheries worldwide. Myxozoans are microparasites that have a complex life cycle, alternating between a vertebrate and an invertebrate host, being able to be responsible for causing diseases mainly in fish, with high mortality rates. Many myxozoans remain unknown to the scientific community and their potential to cause disease in fish remains undetermined in some cases. Thus, the present study aimed to analyze morphologically and molecularly myxozoans parasites of fish from inland rivers of the state of São Paulo, as well as to characterize the phylogenetic relationships of these parasites and their pathological potential. From 2016 to 2019 specimens from five species from Characiformes and Siluriformes orders were collected. Molecular biology, histology, and ultrastructural analysis techniques were used taxonomic description of species. Myxobolus batalhensis n. sp. found parasitizing Salminus hilarii (Bryconidae), Myxobolus imparfinis n. sp. found parasitizing Imparfinis mirini (Heptapteridae), Henneguya sp. 1 found parasitizing Prochilodus lineatus (Prochilodontidae) and Henneguya sp. 2 found parasitizing Astyanax lacustris (Characidae), had the morphological and molecular characterization performed. A supplementary study on Henneguya guanduensis found parasitizing Hoplosternum littorale (Callichthyidae) was done, with molecular, ultrastructural and phylogenetic analysis. A phylogenetic analysis of the myxozoans species of Myxobolidae family parasitizing fish from Brazil was performed. The diversity of myxozoans found in the target fish species of this study highlights the need to intensify the study of the diversity and pathogenic potential of these parasites. (AU)

• FAPESP's process: 15/24901-2 - Phylogeny and taxonomy of myxozoans parasites of fish from inland rivers in the State of São Paulo
• Grantee: Diego Henrique Mirandola Dias Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate