Evaluation the of angiogenesis and inflammatory response in free skin grafts submitted to laser therapy in rats (Rattus novergicus albinus wistar)

Author(s)

Stella Habib Moreira

• Document type: Master's Dissertation
• Press: Jaboticabal. 2019-04-25.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2019-02-28
• Advisor: Andrigo Barboza De Nardi

Abstract

The reconstructive techniques has been widely used in Veterinary Medicine. To reduce inherent complications to the reconstructive techniques, the postoperative adjuvants therapies are used, such as low-level laser therapy (LLLT). The aim of this work is to determine the low-level laser therapy’s effects on the healing, inflammation and vascularization of the free skin grafts grafts in applicable time intervals to the veterinary surgery routine. Forty rats (Rattus novergicus albinus wistar) were used, and each one was submitted to the autogenous free skin graft on the interescapular region. They were allocated randomly in five groups (G1, G2, G3, G4 and G5) according to the 6 J/cm² or 10 J/cm² doses every 3 or 5 days for 15 days. The macroscopic evaluation on the third, seventh and fifteenth day did not show alterations of the LLLT on the grafts. The histochemical evaluation with picrosirius had more expression of the type I collagenous – red in the grafts of G5 (p<0,05), while in the G1 did not; the expression of the type III collagenous – green was not influenced by LPLT. The histochemical evaluation with hematoxylin-eosin showed more numbers of fibroblasts in the grafts of G4 (p<0,05) and less hemorrhage in grafts of G5 p<0,05). It had modulation of the inflammatory response in the irradiated cutaneous grafts, while the LPLT had no effect on the angiogenesis. Then, it observed that the exposure of the cutaneous grafts to the 6 J/cm² or 10 J/cm² doses every 5 days improved the healing and the modulation of the local inflammation. (AU)

• FAPESP's process: 17/25951-9 - Evaluation of angiogenesis and inflammatory response in cutaneous grafts submitted to laser therapy in rats (Rattus novergicus albinus)
• Grantee: Stella Habib Moreira
• Support Opportunities: Scholarships in Brazil - Master